Sulaiman, M.Sc., M.Phil, Ph.D. Microbiological Sciences Branch Southeast Regional Laboratory (SRL) Office of regulatory Affairs (ORA) Atlanta, Georgia Background: • Ph.D. – University of Delhi, India • M.Phil. University, India • M.Sc. Research Interests: Dr.

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Sulaiman joined the Microbiological Sciences Branch, Southeast Regional Laboratory, U. Food and Drug Administration, Atlanta, Georgia as a Research Microbiologist on October 12th of 2008, with over 16 years of research experience and expertise in the field of molecular genetics and its application in method development to detect and differentiate various human-pathogenic emerging infectious agents. Before coming to FDA, Dr. Sulaiman worked at the Centers for Disease Control (CDC) for eleven and half years from 1997 to 2008. Sulaiman obtained his PhD degree in 1992 to study Conservation Biology, Population Genetics and Ecology of Endangered Species from University of Delhi. Research Interests: Prior to joining FDA I conducted research in Cell Culture, Virology, Microbial Genetics and Select Agents. Research interests with FDA include rapid methods development with Select Agents in foods, virus in foods, high throughput molecular assays and methods as well as investigating nano potential for field capable real time food analysis.

Proposed Regulatory Research Project for the FDA Commissioner's Fellow: The goal of the fellowship research project is to develop a portable biosensor platform for environmental and later, food analysis. Novel biosensor concepts are needed to reduce the time to process, weight of current prototype detection platforms and to reduce the quantities of chemicals required to run them. Small foot print biosensors with multiplexing capabilities that are robust and very sensitive will meet these requirements.

Small hand-held lateral flow devices are light but have sensitivity levels ranging from 105 to 107cfu/g. Sensor platforms made from functionally derived electro-spun or melt-spun fibers can be prepared and conformally coated with conductive polymers by oxidative chemical vapor deposition (oCVD) to create electrochemical biosensor devices. The geometry of the melt-spun membrane is such that it creates an extremely high surface area for increased antibody attachment. Our goal is to utilize masking, lithographic or nano-stencil methods to construct grids of small independent “mini-sensors” that individually can detect 1-100 positive receptor/target interactions. Combining thousands or millions of these mini-sensors will allow our biosensor(s) the chance to interact with a single or very low number of pathogens in a very large sample and still report a positive result.

The main objective is to develop small hand held electrochemical field capable detection devices utilizing nonwoven materials coated with conductive polymers. Studies are investigating the choice of the nonwoven membrane material and fiber diameter, the conductive polymer coating, and the process for depositing the conductive polymer onto the membrane. Ongoing studies will identify the best methods to functionalize the conductive membranes with pathogen specific antibodies, determine optimal distribution of Ab and to determine the most effective surface attachment chemistries. Lastly, an electrochemical cell will be designed and constructed in order to convert the antibody-antigen reaction into a measurable detection signal. The completion of an optimized electrochemical cell prototype will allow us to proceed to our final goal of having each “mini-sensor” directly interfaced with a computer for ongoing and instantaneous detection capability. Applicant Requirements: Experience working with nano-materials and particularly CVD, exposure or experience with systems engineering and or advanced degree in a relevant field with a high level of motivation and commitment will be considered.

Selected Recent Publications: Torosian, S.D., Regan, P., Taylor, M. Persistence of Yersinia pestis in Seeded Bottled Water Samples.

Of Micro., 2009, 55:(9) 1125-1129. Torosian, S.D., Regan, P., Doran, T., Taylor, M. A Refrigeration Temperature of 4 °C Does Not Prevent Static Growth of Yersinia Pestis in Heart Infusion Broth. 2009, 55:(9) 1119-1124.

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